Manifold Bio

Scientist II/Senior Scientist, Protein Sciences – Cell Binding Assays

Boston · Senior

Sponsorship not specified$125k-$175kDetected 12 days ago

Full-Stack DevelopmentFlow CytometryMolecular BiologyProtein AnalysisAnimal Studies

About the role

Our team of protein engineers, biologists, and computational scientists works across this full stack to pursue programs both internally and with leading pharma companies.
This is a senior, hands-on role at the intersection of molecule discovery and therapeutic evaluation - you will be the single point of accountability for the ability to understand candidate function in translationally relevant contexts.

Design and generate stable and transient cell lines expressing target antigens (including membrane receptors and other challenging targets) to support antibody discovery and characterization campaigns.
Develop and maintain cell lines optimized for whole-cell panning and display-based selection workflows.
Develop, optimize, and qualify cell-based binding assays (flow cytometry / FACS, cell ELISA, and equilibrium binding formats) to characterize antibody-type molecules across a range of formats.
Proven ability to work independently, design rigorous experiments, troubleshoot complex problems, and interpret data with sound scientific judgment.

PhD in cell biology, immunology, biochemistry, molecular biology, or a related field, with 3+ years of relevant post-graduate experience (or MS with 8+ years) in a biotech/pharma discovery setting.
Expertise in mammalian cell engineering and cell line development..
Demonstrated experience with FACS and general assay development.
Strong practical knowledge of antibody characterization concepts - on-cell affinity/avidity, specificity and selectivity, epitope binning, and competition/blocking assays.
Track record of collaborating cross-functionally and communicating results clearly to project teams.
The ideal candidate brings deep expertise in mammalian cell line development and engineering, bioassay development, and protein-protein interactions.
You are comfortable in a fast-moving startup and flourish in driven, innovative, collaborative environments.

Direct experience with whole-cell panning and display-based selection (phage and/or yeast display), including negative-selection/depletion strategies against native membrane targets.
Familiarity with a range of antibody formats
Experience with orthogonal biophysical binding methods (SPR/BLI, e.g. Biacore or Octet) to complement cell-based data.
Experience adapting assays to higher-throughput or automated platforms.
Final compensation decisions are made using a consistent leveling framework and consider the candidate's experience, interview performance, and expected impact.
This role is eligible for:

Final compensation decisions are made using a consistent leveling framework and consider the candidate's experience, interview performance, and expected impact.
$125,000 - $175,000
Annual performance-based target bonus
Our compensation ranges are reviewed annually to ensure alignment with market trends and internal equity.

Annual performance-based target bonus
Comprehensive medical, dental, and vision coverage
Flexible paid time off and holidays
This reflects the typical offer range for this role, based on experience, role scope, and internal equity.
Perks including on-site gym, onsite lunch, and commuter support

This listing is sourced directly from Manifold Bio's careers page and normalized into a canonical job model.