Profluent
Scientist II, mRNA Research & Production
Emeryville, California, United States; On-site · Mid
Work authorization required$147k-$161kDetected 30 days ago
SparkData AnalysisPharmacologyGMPqPCRCRISPRMolecular BiologyNGSResearchMass SpectrometryProtein AnalysisAnimal StudiesCommunicationMentoring
About the role
- This is a hands-on, lab-based role focused on the synthesis, purification, characterization, and optimization of research-grade mRNA for high-throughput screening, cell-based assays, and scaled production for mouse studies.
- Sponsorship will not be provided now or at any time in the future for this position.
- Hiring Salary Range $147,000 - $161,000 USD
Responsibilities
- Lead and execute end-to-end research-grade automated mRNA production workflows, including template preparation, plasmid DNA sequencing data analysis, linearization, IVT, capping or cap analog workflows, nucleotide modification, purification, buffer exchange, storage, and batch release for research use
- Design mRNA (5'/3' UTR, coding sequence, modifications, etc.) to improve protein yield, activity, and specificity using established and cutting-edge methodology
- Manage and mentor a small mRNA production core function, helping coordinate production schedules, train team members, review batch quality, and maintain reliable material delivery to R&D teams
- Partner with payload engineering, delivery, in vivo pharmacology, bioanalytics, and translational teams to ensure mRNA materials are fit-for-purpose for platform and program needs
- Produce high-quality mRNA for rapid screening of customized base editors in cell-based assays, as well as scaled batches to support mouse studies, assay development, and early translational experiments
- Implement and interpret fit-for-purpose mRNA analytical assays, including concentration, integrity, transcript purity, size distribution, dsRNA, residual DNA, endotoxin, and functional expression or potency readouts
- Maintain clear SOPs, batch records, analytical summaries, sample inventories, and production documentation to support reliable material handoff and reproducible workflows
- Support mRNA production workflows for emerging programmable genome engineering modalities, including engineered recombinases, as Profluent's platform and portfolio evolve
- High-growth opportunity with meaningful impact on the future of protein design
Requirements
- PhD with 2-3 years relevant experience, MS with 10+ years, or BS with 12+ years of relevant experience
- Deep hands-on experience with IVT-based mRNA production and the technical challenges of generating high-quality RNA for in vitro and in vivo studies
- Experience with plasmid or DNA template preparation, linearization, IVT, capping or cap analog workflows, nucleotide modification, mRNA purification, and product characterization
- Strong understanding of mRNA quality attributes, process-related impurities, IVT performance, purification strategies, stability, storage, and the relationship between mRNA quality and biological performance
- Experience producing mRNA for discovery-stage screening and scaling for in vivo preclinical rodent studies
- Experience with modified nucleotides, cap analogs, enzymatic capping, polyA strategies, UTR/codon/design-variable screening, or RNA design-for-potency and manufacturability
- Experience coordinating, training, mentoring, or managing research associates or technical team members in a production, process development, core-lab, or high-throughput R&D environment
- Experience with preparative chromatography, TFF, bead-based purification, buffer exchange, or other mRNA purification approaches
- Strong technical judgment, attention to detail, and ability to independently troubleshoot production and analytical workflows
- Ability to work effectively with cross-functional teams in a fast-paced R&D environment
- PhD, MS, or BS in molecular biology, biochemistry, chemical engineering, bioengineering, biotechnology, pharmaceutical sciences, or a related discipline, with relevant industry experience in mRNA production, RNA process development, nucleic acid manufacturing, or related areas
- Excellent organizational and communication skills, including experience maintaining SOPs, batch records, analytical summaries, and clear technical documentation
Compensation
- Hiring Salary Range $147,000 - $161,000 USD
Benefits
- Competitive compensation package with equity participation
- Comprehensive benefits including health/dental/vision insurance
- Generous PTO policy and commitment to work-life balance
Equal opportunity
- equal opportunity employer promoting diversity and inclusion in the workspace.
Visa & Work Authorization
- Applicants must have ongoing work authorization in the United States that does not require employer sponsorship.
- Sponsorship will not be provided now or at any time in the future for this position.
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