Pivotal Future

Pivotal Future

Senior Research Associate, In Vivo Discovery

Cambridge, Massachusetts · Senior · Full-time

Work authorization required$90k-$120kDetected 43 days ago
PharmacologyBioinformaticsPCRqPCRCRISPRFlow CytometryNGSCell CultureResearchExperimental DesignProtein AnalysisWestern BlottingAnimal StudiesLeadershipCommunicationCollaborationPublic Speaking

About the role

  • The location for this position is onsite, 5 days per week, in our Boston lab.
  • Eligible to work in the United States for any employer without sponsorship.
  • Able to work onsite in the lab located in San Diego.

Responsibilities

  • Design, develop, and qualify molecular assays to measure CRISPR editing outcomes in vitro and in vivo, including amplicon NGS for indel quantification, TIDE/ICE, ddPCR, and orthogonal confirmation methods.
  • Lead cell-based pharmacology assays evaluating delivery efficiency, editing efficiency, and on-target activity of LNP- and antibody-based delivery platforms.
  • Maintain primary and immortalized mammalian cell lines using aseptic technique
  • perform transfection and electroporation of CRISPR reagents.
  • Contribute to study design, protocol development, and SOPs. Mentor junior research associates and support cross-functional collaboration with Delivery, Editing, and Translational teams.
  • Maintain rigorous electronic and paper lab records consistent with reproducibility and data integrity standards.

Requirements

  • BS with 6+ years or MS with 4+ years of hands-on research experience in molecular and cell biology in industry or academia.
  • Direct experience with CRISPR gene editing, including assay readouts for editing efficiency (amplicon NGS, TIDE/ICE, or equivalent).
  • Strong working knowledge of core molecular and analytical methods: PCR, RT-qPCR, Sanger and NGS sequencing, SDS-PAGE / Western blot (or Jess capillary electrophoresis), ELISA, and flow cytometry.
  • Experience designing and executing in vivo rodent studies, including tissue collection and downstream analysis.
  • Ability to independently scope, plan, and execute experiments with minimal supervision
  • track record of contributing to project decisions based on data.
  • Authorization to work in the United States without current or future sponsorship required.
  • Demonstrated expertise in mammalian cell culture and design, execution, and troubleshooting of cell-based assays.
  • Mammalian cell culture, cell transfection, and sterile techniques.
  • Clear written and verbal communication
  • comfortable presenting data to a scientific audience.

Nice to have

  • Hands-on experience with hematopoietic stem cells (HSCs / HSPCs), including isolation, ex vivo expansion, and multilineage differentiation assays (CFU, erythroid, myeloid).
  • Experience with LNP-based delivery of nucleic acids, including formulation handling, dose preparation, and in vivo administration.
  • Experience with antibody-based or receptor-targeted delivery strategies.
  • Familiarity with bioinformatics tools for amplicon sequencing analysis (e.g., CRISPResso, CRISPRessoBatch).
  • Prior experience supporting IND-enabling or GLP studies.

Compensation

  • $90,000 - $120,000

Benefits

  • Competitive salary, equity, and comprehensive benefits package.
  • We offer an exceptional benefits package to include Medical, Dental, Vision, Life, Disability, a 401k Match of 4%, Paid Time Off and Equity.

Company info

  • We are an innovative, stealth-mode biotech company developing in vivo gene editing therapeutics with a focus on targeted delivery of CRISPR machinery to therapeutically relevant cell types.
  • The Translational Research team is hiring a Senior Research Associate to lead the cellular and molecular characterization of delivery and editing platforms supporting our preclinical programs.
  • This is a hands-on bench role with significant ownership of experimental design, execution, and data interpretation.
  • We are an Equal Opportunity Employer.

Equal opportunity

  • Employer
  • We are an Equal Opportunity Employer. We celebrate diversity and are committed to creating an inclusive environment for all employees. All employment decisions are based on business needs, job requirements, and individual qualifications, without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran, or disability status.
  • We celebrate diversity and are committed to creating an inclusive environment for all employees.
  • All employment decisions are based on business needs, job requirements, and individual qualifications, without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, veteran, or disability status.

Visa & Work Authorization

  • Eligible to work in the United States for any employer without sponsorship.
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This listing is sourced directly from Pivotal Future's careers page and normalized into a canonical job model.