AstraZeneca

AstraZeneca

Senior Scientist, Cell Line Development

US - Gaithersburg - MD · Senior

Sponsorship not specified$112k-$168kDetected 3 days ago
Data AnalysisqPCRCRISPRFlow CytometryMolecular BiologyProtein AnalysisCollaboration

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odds of building a lasting career here

59Sponsors, lottery-bound
Cap-exempt (no lottery)0
Sponsors this role100
Entry-level history0
PERM / green-card track0
Lottery odds (Level III)83
Fits your clock70

Sponsors, but it's cap-subject — you still face the weighted lottery (~45% per draw at Level III). Good if you win; have a cap-exempt backup on your list.

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About the role

  • This position is based in Gaithersburg, MD.
  • The annual base pay for this position ranges from $ 112,304.00 - 168,456.00 The annual base pay for this position ranges from $112,304.00 - $168,456.00.
  • Our positions offer eligibility for various incentives-an opportunity to receive short-term incentive bonuses, equity-based awards for salaried roles and commissions for sales roles.

Responsibilities

  • Cell Line Development & Viral Packaging: Perform mammalian CLD activities, including single-cell and mini-pool generation, small-scale viral vector packaging and high throughput titration, and clone selection to achieve robust viral productivity for upstream processes.
  • partner with analytical and process development teams to link clone selection choices to critical quality attributes using molecular/cell analytics (e.g., flow cytometry, qPCR/ddPCR, ELISA, titering assays).
  • Cell line engineering: lead and implement cell line engineering to improve cell line performance, including LVV packaging efficiency, vector quality, genetic stability and overall robustness
  • Data Analysis & Collaboration: Analyze and interpret complex datasets, maintain accurate experimental documentation, and collaborate with upstream Process Development and Analytical teams to support smooth process handoffs.
  • High-Throughput Screening & Analytics Interface: Establish efficient, scalable workflows for rapid cell characterization and clone evaluation, and high-throughput titer screening; partner with analytical and process development teams to link clone selection choices to critical quality attributes using molecular/cell analytics (e.g., flow cytometry, qPCR/ddPCR, ELISA, titering assays).
  • Your work will center on independently accomplishing single-cell isolation, small-scale viral packaging, high-throughput screening, and evaluating clone productivity and product quality to deliver stable and top-performing clones supporting AstraZeneca's emerging Cell Therapy portfolio.
  • Perform mammalian CLD activities, including single-cell and mini-pool generation, small-scale viral vector packaging and high throughput titration, and clone selection to achieve robust viral productivity for upstream processes.
  • Establish efficient, scalable workflows for rapid cell characterization and clone evaluation, and high-throughput titer screening; partner with analytical and process development teams to link clone selection choices to critical quality attributes using molecular/cell analytics (e.g., flow cytometry, qPCR/ddPCR, ELISA, titering assays).
  • lead and implement cell line engineering to improve cell line performance, including LVV packaging efficiency, vector quality, genetic stability and overall robustness
  • Analyze and interpret complex datasets, maintain accurate experimental documentation, and collaborate with upstream Process Development and Analytical teams to support smooth process handoffs.

Requirements

  • Core Technical Expertise:
  • Proficiency in single-cell isolation using cell sorter, advanced imaging systems, and clone characterization via flow cytometry.
  • Experience with high-throughput titration assays and utilizing, maintaining, and troubleshooting automated liquid handling and high-throughput screening platforms.
  • Experience with stable genetic modification of mammalian cells using approaches such as CRISPR/cas9
  • Experience in monoclonality validation and expanding stable master cell lines.
  • Hands-on experience in virus/vector engineering for host cell line optimization or vector performance improvement.
  • Education: PhD degree or Master's degree (or equivalent to 3-4 years of experience) in Cell Biology, Molecular Biology, Bioengineering, Virology, or a related scientific field.
  • Extensive, hands-on experience in mammalian CLD, viral vector packaging, and clone screening, with a strong emphasis on cultivating and optimizing HEK293 based host cells in suspension culture for high-yield Lentiviral vector production.
  • Desirable Experience:
  • Background in viral vector biology, cell and gene therapy within the pharmaceutical industry, supporting vector design/engineering for maximized titers, quality and overall manufacturability.
  • Viral vector stable producer cell line development
  • Core Competencies: Effective interpersonal skills, meticulous attention to detail, and independent troubleshooting capabilities and in a fast-paced laboratory environment.
  • The annual base pay for this position ranges from $
  • 112,304.00 - 168,456.00

Compensation

  • The annual base pay for this position ranges from $

Benefits

  • offered include qualified retirement programs, paid time off (i.e., vacation, holiday, and leaves), as well as health, dental, and vision coverage in accordance with the terms of the applicable plans.

Equal opportunity

  • If you have a disability or special need that requires accommodation, please complete the corresponding section in the application form.
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This listing is sourced directly from AstraZeneca's careers page and normalized into a canonical job model.