Legend Biotech US
Molecular Biology Scientist/Senior Associate Scientist
Philadelphia, PA · Senior
Sponsorship not specifiedDetected 26 days ago
qPCRCRISPRFlow CytometryMolecular BiologyNGSCell CultureResearchProtein AnalysisWestern BlottingCommunicationCollaborationProblem SolvingAdaptability
About the role
- Legend Biotech is a global biotechnology company dedicated to treating, and one day curing, life-threatening diseases.
- From our three R&D sites around the world, we apply these innovative technologies to pursue the discovery of safe, efficacious and cutting-edge therapeutics for patients worldwide.
- This role emphasizes reliable experimental execution, timely delivery of materials, and clear data communication.
Responsibilities
- Execute viral vector plasmid and helper plasmid design and construction to support gene overexpression, knock-down, and knock-out studies independently.
- Perform routine molecular cloning workflows and ensure plasmid quality meets established QC standards for vector production.
- Perform mammalian cell culture and transient transfection under BSL-2 conditions.
- Support small-scale vector production using suspension cell lines for early development activities.
- Analyze data, summarize results, present clear experiment conclusions, escalate issues, and propose next steps to project leads.
- Legend Biotech entered into a global collaboration agreement with Janssen, one of the pharmaceutical companies of Johnson & Johnson, to jointly develop and commercialize ciltacabta gene autolecuel (cilta-cel).
Requirements
- Scientist: M.S. with 5+ years or Ph.D. with 0-2 years in a related field
- hands-on molecular cloning, cell engineering and mammalian cell culture required.
- Familiarity with viral vector process development concepts (upstream and/or downstream).
Nice to have
- Hands-on experience in molecular biology techniques (cloning, plasmid prep, sequence analysis and construct verification).
- Experience with mammalian cell culture and transient transfection
- familiarity with BSL-2 practices and documentation.
- Experience generating engineered cell lines (stable expression and/or CRISPR KO/KI) including cloning and basic screening (Scientist preferred).
- Exposure to viral vector production and/or viral vector workflows (LVV preferred).
- Experience with titering and functional readouts (e.g., transduction efficiency and gene expression).
- Experience with qPCR/dPCR, flow cytometry, Western blot, and ELISA for tittering and functional assays.
- Strong ELN documentation, sample tracking, and ability to follow protocols/SOPs with attention to detail.
Skills
- Execute molecular/cellular assays to evaluate vector performance using qPCR/dPCR, ELISA/Western Blot, and flow cytometry.
- Adhere to BSL-2 biosafety standards and Legend policies, maintaining accurate and compliant electronic lab notebooks and documentation.
- Collaborate cross-functional support tech transfer activities as needed.
- Independently plans day-to-day execution within project goals, recommends optimizations and next experiments.
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